Recombinant人BRCA2 protein (ab112253)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionP51587
    • 种属Human
    • 序列SPQMTPFKKFNEISLLESNSIADEELALINTQALLSGSTGEKQFISVSES TRTAPTSSEDYLRLKRRCTTSLIKEQESSQASTEECEKNKQDTITTKKYI
    • 分子量37 kDa including tags
    • 氨基酸3319 to 3418
    • 标签GST tag N-Terminus

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技术指标

Our Abpromise guarantee covers the use of ab112253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

    ELISA

  • 形式Liquid
  • 补充说明Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • BRCA 2
    • BRCA1/BRCA2 containing complex subunit 2
    • Brca2
    • BRCA2, DNA repair associated
    • BRCA2_HUMAN
    • BRCC 2
    • BRCC2
    • Breast and ovarian cancer susceptibility gene early onset
    • breast and ovarian cancer susceptibility protein 2
    • Breast cancer 2 early onset
    • Breast Cancer 2 tumor suppressor
    • Breast cancer susceptibility protein BRCA2
    • Breast cancer type 2 susceptibility protein
    • BROVCA2
    • FACD
    • FAD
    • FAD 1
    • FAD1
    • FANCB
    • FANCD
    • FANCD 1
    • FANCD1
    • FANCD1 gene
    • Fanconi anemia complementation group D1
    • Fanconi anemia group D1 protein
    • GLM3
    • mutant BRCA2
    • OTTHUMP00000018803
    • OTTHUMP00000042401
    • PNCA2
    • XRCC11
    see all
  • 功能Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures.
  • 组织特异性Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.
  • 疾病相关Defects in BRCA2 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
    Defects in BRCA2 are the cause of pancreatic cancer type 2 (PNCA2) [MIM:613347]. It is a malignant neoplasm of the pancreas. Tumors can arise from both the exocrine and endocrine portions of the pancreas, but 95% of them develop from the exocrine portion, including the ductal epithelium, acinar cells, connective tissue, and lymphatic tissue.
    Defects in BRCA2 are a cause of susceptibility to breast-ovarian cancer familial type 2 (BROVCA2) [MIM:612555]. A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate.
    Defects in BRCA2 are the cause of Fanconi anemia complementation group D type 1 (FANCD1) [MIM:605724]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
    Defects in BRCA2 are a cause of glioma type 3 (GLM3) [MIM:613029]. Gliomas are benign or malignant central nervous system neoplasms derived from glial cells. They comprise astrocytomas and glioblastoma multiforme that are derived from astrocytes, oligodendrogliomas derived from oligodendrocytes and ependymomas derived from ependymocytes.
  • 序列相似性Contains 8 BRCA2 repeats.
  • 翻译后修饰Phosphorylated by ATM upon irradiation-induced DNA damage.
    Ubiquitinated in the absence of DNA damage; this does not lead to proteasomal degradation. In contrast, ubiquitination in response to DNA damage leads to proteasomal degradation.
  • Information by UniProt

Recombinant Human BRCA2 protein 图像

  • Coomassie blue stained 12.5% SDS page analysis of ab112253

Recombinant Human BRCA2 protein (ab112253)参考文献

ab112253 has not yet been referenced specifically in any publications.

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