Recombinant人Borealin/CDCA8 protein (ab107144)

概述

  • 产品名称
    Recombinant人Borealin/CDCA8 protein
  • 蛋白长度
    Protein fragment

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MGSSHHHHHHSSGLVPRGSHMRKLASFLKDFDREVEIRIKQIESDRQNLL KEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEIN KLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPP SKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVF KTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQ RHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK
    • 分子量
      32 kDa including tags
    • 氨基酸
      19 to 280
    • 标签
      His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab107144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Mass Spectrometry

    SDS-PAGE

  • 质谱法
    MALDI-TOF
  • 纯度
    > 90 % SDS-PAGE.
    Purified by using conventional chromatography techniques.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.2M Sodium chloride, 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 8.0

常规信息

  • 别名
    • BOR
    • BOREA_HUMAN
    • Borealin
    • CDCA8
    • Cell division cycle associated 8
    • cell division cycle associated protein 8
    • Cell division cycle-associated protein 8
    • Dasra B
    • Dasra-B
    • DasraB
    • FLJ10468
    • FLJ12042
    • hDasra B
    • hDasra-B
    • MESRGP
    • OTTHUMP00000004514
    • pluripotent embryonic stem cell related gene 3 protein
    • Pluripotent embryonic stem cell-related gene 3 protein
    see all
  • 功能
    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
  • 序列相似性
    Belongs to the borealin family.
  • 发展阶段
    Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.
  • 结构域
    The C-terminal region (aa 207-280) represents the dimerization motif.
  • 翻译后修饰
    Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
    Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
  • 细胞定位
    Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
  • Information by UniProt

图片

  • ab107144 at 3 µg analysed by 15% SDS PAGE.

文献

This product has been referenced in:

See 1 Publication for this product

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