重组人BMP2蛋白(Active) (ab50099)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: HPLC, Cellular Activation, SDS-PAGE
描述
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产品名称
重组人BMP2蛋白(Active)
参阅全部 BMP2 蛋白酶 -
生物活性
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells.
The expected ED50 for this effect is 0.5-1.0 μg/ml.
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纯度
> 98 % SDS-PAGE.
>98% by HPLC analyses. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR -
预测分子量
26 kDa -
氨基酸
283 to 396 -
额外的序列信息
Homodimeric protein consisting of two 115 amino acid polypeptide chains. Full length mature protein, minus the signal peptide and the propeptide.
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技术指标
Our Abpromise guarantee covers the use of ab50099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
HPLC
Cellular Activation
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Centrifuge the vial prior to opening. Reconstitute in water containing BSA (50ug of BSA per 1ug of protein) to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
常规信息
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别名
- BDA2
- BMP-2
- BMP-2A
see all -
功能
Induces cartilage and bone formation. -
组织特异性
Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. -
序列相似性
Belongs to the TGF-beta family. -
细胞定位
Secreted. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab50099 被引用在 2 文献中.
- Zhang W et al. Preparation of a nanopearl powder/C-HA (chitosan-hyaluronic acid)/rhBMP-2 (recombinant human bone morphogenetic protein-2) composite artificial bone material and a preliminary study of its effects on MC3T3-E1 cells. Bioengineered 13:14368-14381 (2022). PubMed: 35758269
- Xie YF et al. Pulsed electromagnetic fields stimulate osteogenic differentiation and maturation of osteoblasts by upregulating the expression of BMPRII localized at the base of primary cilium. Bone 93:22-32 (2016). PubMed: 27622883