Recombinant人Bmi1 protein (ab114169)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionP35226
    • 种属Human
    • 序列MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETS KYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAA HPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKD KEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPL KDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELE SDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSP SGNHQSSFANRPRKASVNGSSATSSG
    • 分子量62 kDa including tags
    • 氨基酸1 to 326

技术指标

Our Abpromise guarantee covers the use of ab114169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    ELISA

    SDS-PAGE

  • 形式Liquid
  • 补充说明Protein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • B lymphoma Mo MLV insertion region (mouse)
    • B lymphoma Mo MLV insertion region 1 homolog
    • Bmi 1
    • BMI1
    • BMI1 polycomb ring finger oncogene
    • BMI1_HUMAN
    • Flvi 2/bmi 1
    • FLVI2/BMI1
    • MGC12685
    • Murine leukemia viral (bmi 1) oncogene homolog
    • Oncogene BMI 1
    • PCGF 4
    • PCGF4
    • Polycomb complex protein BMI 1
    • Polycomb complex protein BMI-1
    • Polycomb group protein Bmi1
    • Polycomb group ring finger 4
    • Polycomb group RING finger protein 4
    • RING finger protein 51
    • RNF 51
    • RNF51
    see all
  • 功能Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
  • 序列相似性Contains 1 RING-type zinc finger.
  • 翻译后修饰Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
  • 细胞定位Nucleus. Cytoplasm.
  • Information by UniProt

Recombinant Human Bmi1 protein 图像

  • ab114169 on a 12.5% SDS-PAGE Stained with Coomassie Blue.
  • Anti-Bmi1 antibody (ab63767) at 2 µg/ml + Recombinant Human Bmi1 protein (ab114169) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

Recombinant Human Bmi1 protein (ab114169)参考文献

ab114169 has not yet been referenced specifically in any publications.

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