Recombinant人BLNK protein (ab104120)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ8WV28
    • 种属Human
    • 序列MGSSHHHHHH SSGLVPRGSH MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMNKIKKLK VKAPPSVPRR DYASESPADE EQQWSDDFDS DYENPDEHSD SEMYVMPAEE NADDSYEPPP VEQETRPVHP ALPFARGEYI DNRSSQRHSP PFSKTLPSKP SWPSEKARLT STLPALTALQ KPQVPPKPKG LLEDEADYVV PVEDNDENYI HPTESSSPPP EKAPMVNRST KPNSSTPASP PGTASGRNSG AWETKSPPPA APSPLPRAGK KPTTPLKTTP VASQQNASSV CEEKPIPAER HRGSSHRQEA VQSPVFPPAQ KQIHQKPIPL PRFTEGGNPT VDGPLPSFSS NSTISEQEAG VLCKPWYAGA CDRKSAEEAL HRSNKDGSFL IRKSSGHDSK QPYTLVVFFN KRVYNIPVRF IEATKQYALG RKKNGEEYFG SVAEIIRNHQ HSPLVLIDSQ NNTKDSTRLK YAVKVS
    • 分子量53 kDa including tags
    • 氨基酸1 to 456
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab104120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF
  • 纯度> 90 % SDS-PAGE.
    Purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, 1mM DTT, 0.1mM PMSF, pH 8

常规信息

  • 别名
    • AGM4
    • B cell adapter containing SH2 domain protein
    • B cell adapter containing Src homology 2 domain protein
    • B cell adaptor containing SH2 domain
    • B cell linker
    • B cell linker protein
    • B cell-specific adaptor protein
    • B-cell activation
    • B-cell adapter containing a SH2 domain protein
    • B-cell adapter containing a Src homology 2 domain protein
    • B-cell linker protein
    • BASH
    • Bca
    • BLNK
    • BLNK s
    • BLNK_HUMAN
    • Cytoplasmic adapter protein
    • Ly 57
    • Ly57
    • Lymphocyte antigen 57
    • Lyw 57
    • Lyw57
    • MGC111051
    • SH2 domain-containing leukocyte protein, 65-KD
    • SLP 65
    • SLP-65
    • SLP65
    • Src homology [SH2] domain-containing leukocyte protein of 65 kD
    • Src homology 2 domain containing leukocyte protein of 65 kDa
    • Src homology 2 domain-containing leukocyte protein of 65 kDa
    see all
  • 功能Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis.
  • 组织特异性Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
  • 疾病相关Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
  • 序列相似性Contains 1 SH2 domain.
  • 翻译后修饰Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
  • 细胞定位Cytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction.
  • Information by UniProt

Recombinant Human BLNK protein 图像

  • 15% SDS-PAGE analysis of ab104120 (3 µg).

Recombinant Human BLNK protein (ab104120)参考文献

ab104120 has not yet been referenced specifically in any publications.

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