Recombinant人Bcl-2 protein (ab59853)



  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP10415
    • 种属Human
    • 氨基酸1 to 218


Our Abpromise guarantee covers the use of ab59853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用


  • 纯度> 95 % SDS-PAGE.
    Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
  • 形式Lyophilised
  • Concentration information loading...


  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 250mM Sodium chloride, 10mM Tris HCl, 1mM EDTA, pH 8

  • 复溶Resuspend product in 100ul of 10% Acetic acid (Glacial), over night at 4°C.


  • 别名
    • Apoptosis regulator Bcl 2
    • Apoptosis regulator Bcl-2
    • Apoptosis regulator Bcl2
    • AW986256
    • B cell CLL/lymphoma 2
    • B cell leukemia/lymphoma 2
    • Bcl-2
    • Bcl2
    • BCL2_HUMAN
    • C430015F12Rik
    • D630044D05Rik
    • D830018M01Rik
    • Leukemia/lymphoma, B-cell, 2
    • Oncogene B-cell leukemia 2
    • PPP1R50
    • Protein phosphatase 1, regulatory subunit 50
    see all
  • 功能Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
  • 组织特异性Expressed in a variety of tissues.
  • 疾病相关A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
  • 序列相似性Belongs to the Bcl-2 family.
  • 结构域BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
    The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
    The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
  • 翻译后修饰Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
    Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
  • 细胞定位Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt

Recombinant Human Bcl-2 protein (ab59853)参考文献

ab59853 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab59853.
Please use the links above to contact us or submit feedback about this product.