Recombinant人BAFF Receptor protein (Fc Chimera) (ab83931)

概述

描述

  • 性质Recombinant
  • 来源HEK 293 cells
  • 氨基酸序列
    • AccessionQ96RJ3
    • 种属Human
    • 序列Theoretical Sequence: SLRGRDAPAPTPCVPAECFDLLVRHCVACGLLRTPRPKPAGASSPAPRTA LQPQESVGAGAGEAALPGSSNTKVDKKVEPKSCDKTHTCPPCPAPELLGG PSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNA KTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTIS KAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQP ENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYT QKSLSLSPGK
    • 额外的序列信息A fusion of the signal peptide of human GH receptor to the extracellular domain of human BAFF Receptor (aa 2-73), and the Fc region of human IgG1 (aa 93-330), expressed in modified human 293 cells.

技术指标

Our Abpromise guarantee covers the use of ab83931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    The ED50 of BAFF Receptor – Fc Chimera is typically 0.02-0.08 μg/ml as measured by its ability to neutralize BAFF-mediated proliferation of the RPMI 8226 cell line.

  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

常规信息

  • 别名
    • B cell activating factor receptor
    • B-cell-activating factor receptor
    • BAFF R
    • BAFF receptor
    • BAFF-R
    • BAFFR
    • BLyS receptor 3
    • BlySR3
    • BR 3
    • BR3
    • BROMIX
    • CD 268
    • CD268
    • CD268 antigen
    • CVID4
    • MGC138235
    • OTTHUMP00000028746
    • Prolixin
    • TNFRSF 13C
    • Tnfrsf13c
    • TR13C_HUMAN
    • Tumor necrosis factor receptor subunit member 13C
    • Tumor necrosis factor receptor superfamily member 13C
    see all
  • 功能B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response.
  • 组织特异性Highly expressed in spleen and lymph node, and in resting B-cells. Detected at lower levels in activated B-cells, resting CD4+ T-cells, in thymus and peripheral blood leukocytes.
  • 疾病相关Defects in TNFRSF13C are the cause of immunodeficiency common variable type 4 (CVID4) [MIM:613494]; also called antibody deficiency due to BAFFR defect. CVID4 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • 序列相似性Contains 1 TNFR-Cys repeat.
  • 细胞定位Membrane.
  • Information by UniProt

Recombinant human BAFF Receptor protein (Fc Chimera) 图像

  • Lane 1 – MW markers; Lane 2 – ab83931; Lane 3 – ab83931 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83931 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 3 μg of protein was loaded per lane. Gels were stained with Coomassie G250.
    Drop in MW after treatment with PNGase F indicates the presence of N-linked glycans. A subsequent drop in MW after treatment with a glycosidase cocktail indicates O-linked glycans are also present. Additional high MW bands in lane 4 are glycosidase enzymes.
  • A sample of ab83931 without carrier protein was reduced and alkylated. 40 μg of protein was loaded, focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train (Deep Purple™ stained) indicates presence of multiple glycoforms of BAFF Receptor - Fc Chimera. Spots within the spot train were cut from the gel and identified by protein mass fingerprinting as BAFF Receptor - Fc Chimera.
  • Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.

Recombinant human BAFF Receptor protein (Fc Chimera) (ab83931)参考文献

ab83931 has not yet been referenced specifically in any publications.

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