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Our Abpromise guarantee covers the use of ab42596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity: 12 pmol/min/μg.
Activity was tested using 1 μM MEK1 (K97R) as substrate. Assay was performed in a 50 μl reation in 10 mM Tris, pH 7.4, 5 mM
MgCl2 , 0.5 mM EDTA, 0.5 mM DTT, 5 μM ATP, 5 μg MEK1 (K97R) as substrate, and B Raf. 62 ng of B Raf enzyme phosphorylated over 18% of 1 μM MEK1 at room temperature in 1 hr.
Coexpressed in Baculovirus infected Sf9 cell expression system.
Used for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 1.53% Imidazole
Constituents: 0.018% Potassium chloride, 0.0462% DTT, 0.7% Tris HCl, 10% Glycerol, 0.72% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
10% SDS-PAGE with coomassie staining.
Left lane: 4 µg B Raf
Right lane: Protein marker
ab42596 has not yet been referenced specifically in any publications.