Recombinant人Aurora B protein (ab51435)



  • 性质
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属
    • 标签
      GST tag N-Terminus


Our Abpromise guarantee covers the use of ab51435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies


    Western blot

  • 形式
  • 补充说明
    ab51435 was expressed in Sf9 insect cells.

    ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...


  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.


  • 别名
    • AIK2
    • AIM-1
    • AIM1
    • ARK-2
    • ARK2
    • AurB
    • AURKB
    • Aurora 1
    • Aurora and Ipl1 like midbody associated protein 1
    • Aurora kinase B
    • Aurora related kinase 2
    • Aurora- and Ipl1-like midbody-associated protein 1
    • Aurora-B
    • Aurora-related kinase 2
    • Aurora/IPL1 related kinase 2
    • Aurora/IPL1-related kinase 2
    • IPL1
    • PPP1R48
    • Protein phosphatase 1 regulatory subunit 48
    • Serine/theronine kinase 12
    • Serine/threonine protein kinase 12
    • Serine/threonine-protein kinase 12
    • Serine/threonine-protein kinase aurora-B
    • STK-1
    • STK1
    • STK12
    • STK5
    see all
  • 功能
    May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
  • 组织特异性
    High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
  • 疾病相关
    Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
  • 序列相似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
  • 细胞定位
    Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
  • Information by UniProt

Recombinant human Aurora B protein 图像

  • Kinase activity assay - specific activity 190nmol/min/mg
  • Recombinant human Aurora B (tagged). Molecular weight 68 kDa.

Recombinant human Aurora B protein (ab51435)参考文献

ab51435 has not yet been referenced specifically in any publications.

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