Recombinant人Aurora A protein (ab42595)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属
      Human

技术指标

Our Abpromise guarantee covers the use of ab42595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    120 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30?C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.5 mM ATP, 4 uM aurora A substrate, and 0.4 ug/ml Aurora A kinase.
  • 应用

    Inhibition Assay

  • 纯度
    > 90 % SDS-PAGE.

  • 形式
    Liquid
  • 补充说明
    Expressed in Baculovirus infected Sf9 cell expression system.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • AIK
    • ARK-1
    • ARK1
    • AURA
    • Aurka
    • Aurora 2
    • Aurora A
    • Aurora kinase A
    • Aurora-related kinase 1
    • Aurora/IPL1 like kinase
    • Aurora/IPL1-related kinase 1
    • AURORA2
    • Breast tumor-amplified kinase
    • BTAK
    • hARK1
    • IAK
    • IPL1 related kinase
    • MGC34538
    • OTTHUMP00000031340
    • OTTHUMP00000031341
    • OTTHUMP00000031342
    • OTTHUMP00000031343
    • OTTHUMP00000031344
    • OTTHUMP00000031345
    • OTTHUMP00000166071
    • OTTHUMP00000166072
    • PPP1R47
    • Protein phosphatase 1, regulatory subunit 47
    • Serine/threonine kinase 15
    • Serine/threonine kinase 6
    • Serine/threonine-protein kinase 15
    • Serine/threonine-protein kinase 6
    • Serine/threonine-protein kinase aurora-A
    • STK15
    • STK6
    • STK6_HUMAN
    • STK7
    see all
  • 功能
    Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
  • 组织特异性
    Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
  • 序列相似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
  • 细胞定位
    Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
  • Information by UniProt

Recombinant human Aurora A protein (ab42595)参考文献

ab42595 has not yet been referenced specifically in any publications.

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