Recombinant人Apolipoprotein A I (ab50239)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Human
    • 序列
      MDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ

技术指标

Our Abpromise guarantee covers the use of ab50239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Sandwich ELISA

  • 内毒素水平
    < 0.100 Eu/µg
  • 纯度
    > 95 % SDS-PAGE.
    ab50239 purity is greater than 97% by SDS-PAGE gel and HPLC analyses.
  • 形式
    Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).

  • 复溶
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

常规信息

  • 别名
    • Apo-AI
    • ApoA I
    • ApoA-I
    • APOA1
    • APOA1_HUMAN
    • Apolipoprotein A-I(1-242)
    • Apolipoprotein A1
    • Apolipoprotein AI
    • Brp14
    • Ltw1
    • Lvtw1
    • Sep1
    • Sep2
    see all
  • 功能
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 组织特异性
    Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
  • 疾病相关
    Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
    Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
    Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
    Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • 序列相似性
    Belongs to the apolipoprotein A1/A4/E family.
  • 翻译后修饰
    Palmitoylated.
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位
    Secreted.
  • Information by UniProt

图片

  • Standard curve for Apolipoprotein A I (Analyte: ab50239); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab20918 at 1µg/ml and Detector Antibody ab64308 at 0.5µg/ml.

文献

This product has been referenced in:
  • Cubedo J  et al. A novel truncated form of apolipoprotein A-I transported by dense LDL is increased in diabetic patients. J Lipid Res 56:1762-73 (2015). Read more (PubMed: 26168996) »

See 1 Publication for this product

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We have antibodies that are tested and guaranteed for IHC-Fr and ICC which are the main applictaions for indirect immunofluorescence:

ab52945

http://www.abcam.com/index.html?datasheet=52945 ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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