Recombinant人AOS1+UBA2 protein (Chemical Free) (ab80730)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab80730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Conjugation

    SDS-PAGE

  • 纯度> 90 % SDS-PAGE.

  • 形式Lyophilised
  • 补充说明

    Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 50mM HEPES, 200mM Sodium chloride, 1mM DTT, pH 8.0

常规信息

  • 别名
    • SAE1
    • SAE2
    • SUMO 1 activating enzyme subunit 1
    • SUMO1 activating enzyme subunit 2
    • Ubiquitin-like 1-activating enzyme E1A
    • Ubiquitin-like 1-activating enzyme E1B
    • UBLE1A
    • UBLE1B
    see all
  • 相关性E1-SUMO protein is a heterodimer consisted of human AOS1 and UBA2. The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
  • 细胞定位Nuclear

Recombinant Human AOS1+UBA2 protein (Chemical Free) (ab80730)参考文献

ab80730 has not yet been referenced specifically in any publications.

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