Recombinant人Annexin V protein (ab89493)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Human
    • 序列
      ab89493 is purified using conventional chromatography techniques.

技术指标

Our Abpromise guarantee covers the use of ab89493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.

  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

常规信息

  • 别名
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PLACENTAL PROTEIN 4
    • Pp4
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all
  • 功能
    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • 疾病相关
    Pregnancy loss, recurrent, 3
  • 序列相似性
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • 结构域
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻译后修饰
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt

图片

  • Anti-Annexin V antibody (ab30941) at 1 µg/ml + Recombinant Human Annexin V protein (ab89493) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds
  • 15% SDS-PAGE showing ab89483 at approximately 35.9kDa (3µg).

文献

This product has been referenced in:
  • Haedicke K  et al. Lowering photosensitizer doses and increasing fluences induce apoptosis in tumor bearing mice. Biomed Opt Express 7:2641-9 (2016). IF ; Mouse . Read more (PubMed: 27446695) »

See 1 Publication for this product

客户评价及客户问答

Thank you for your inquiry.

In principle the Annexin V protein (ab89493) should be un-bound since we have not used any calcium or phospholipid in our purification process. However, we use unspecified LB when growing the bacteria that express ...

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Thank you for your inquiry. Unfortunately, we currently do not have the molar extinction coefficient available. However, using the amino acid sequence (UniProt ID P08758) and a tool for the prediction of physico-chemical parameters (http://exp...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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