The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生物活性Activates ERK phosphorylation in THP-1 cells.
内毒素水平< 0.100 Eu/µg
% SDS-PAGE. ab108558 is 0.2 µm filtered.
补充说明Working aliquots are stable for up to 3 months when stored at -20°C.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Angiopoietin like 4
Angiopoietin related protein 4
Angiopoietin-like protein 4
Angiopoietin-related protein 4
Fasting induced adipose factor
Hepatic angiopoietin related protein
Hepatic fibrinogen/angiopoietin related protein
Hepatic fibrinogen/angiopoietin-related protein
Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein
PPARG angiopoietin related protein
Weakly similar to angiopoietin 1 [H.sapiens]
功能Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.
组织特异性Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney.
序列相似性Contains 1 fibrinogen C-terminal domain.
细胞定位Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4.
ERK phosphorylation induced by ab108558 (hANGPTL4 - coiled-coil domain) in THP-1 cells.
A. THP-1 monocyte cells were serum starved for 16 hr and then stimulated with ab108558 (500 ng/ml) for 1, 2 and 3 hrs, respectively. Antibodies against pERK1/2
and total ERK1/2 were used for immunoblotting.
B. Cells were treated with 20 µM PD98058 (MEK inhibitor) for 30 min prior adding of 500 ng/ml ab108558. After 3 hrs of incubation, Western blot was performed.
Recombinant human Angiopoietin-like 4 protein (ab108558)参考文献
has not yet been referenced specifically in any publications.