概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP15514
    • 种属Human
    • 序列SVRVEQVVKPPQNKTESENTSDKPKRKKKGGKNGKNRRNRKKKNPCNAEF QNFCIHGECKYIEHLEAVTCKCQQEYFGERCGEKSMKTHSMIDSSLSK
    • 分子量11 kDa
    • 氨基酸101 to 198

技术指标

Our Abpromise guarantee covers the use of ab104355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml.
  • 应用

    SDS-PAGE

    Functional Studies

    Western blot

  • 内毒素水平< 0.100 Eu/µg
  • 纯度> 95 % SDS-PAGE.
    Purity is > 95% by SDS-PAGE gel and HPLC analyses. Sterile filtered through a 0.2 micron filter.
  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.

    Preservative: None
    Constituents: 5mM Sodium phosphate, pH 7.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

常规信息

  • 别名
    • A REG
    • Amphiregulin
    • Amphiregulin B
    • AR
    • AREG
    • AREG_HUMAN
    • AREGB
    • Colorectum cell derived growth factor
    • Colorectum cell-derived growth factor
    • CRDGF
    • MGC13647
    • Schwannoma derived growth factor
    • SDGF
    see all
  • 功能Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells.
  • 序列相似性Belongs to the amphiregulin family.
    Contains 1 EGF-like domain.
  • 细胞定位Membrane.
  • Information by UniProt

Recombinant human Amphiregulin protein 图像

  • Anti-Amphiregulin antibody [MM0089-8K16] (ab89119) at 1/500 dilution + Recombinant human Amphiregulin protein (ab104355) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

Recombinant human Amphiregulin protein (ab104355)参考文献

This product has been referenced in:
  • Hobor S  et al. TGFa and amphiregulin paracrine network promotes resistance to EGFR blockade in colorectal cancer cells. Clin Cancer Res 20:6429-38 (2014). Read more (PubMed: 24916700) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"