Recombinant人alpha A Crystallin protein (ab113189)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP02489
    • 种属Human
    • 分子量20 kDa including tags
    • 氨基酸1 to 173
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab113189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    ab11318 is purified by multi-step chromatography.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.61% Tris, 0.08% DTT, 0.29% Sodium chloride

常规信息

  • 别名
    • Acry 1
    • Alpha crystallin A chain
    • Alpha-crystallin A chain
    • CRYA 1
    • CRYA1
    • CRYAA
    • CRYAA_HUMAN
    • Crystallin alpha 1
    • Crystallin alpha A
    • Heat shock protein beta 4
    • Heat shock protein beta-4
    • HSPB 4
    • HspB4
    • short form
    • Zonular Central Nuclear Cataract
    see all
  • 功能May contribute to the transparency and refractive index of the lens.
  • 疾病相关Defects in CRYAA are a cause of cataract autosomal dominant (ADC) [MIM:604219]. Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood.
  • 序列相似性Belongs to the small heat shock protein (HSP20) family.
  • 翻译后修饰O-glycosylated; contains N-acetylglucosamine side chains.
    Deamidation of Asn-101 in lens occurs mostly during the first 30 years of age, followed by a small additional amount of deamidation (approximately 5%) during the next approximately 38 years, resulting in a maximum of approximately 50% deamidation during the lifetime of the individual.
    Phosphorylation on Ser-122 seems to be developmentally regulated. Absent in the first months of life, it appears during the first 12 years of human lifetime. The relative amount of phosphorylated form versus unphosphorylated form does not change over the lifetime of the individual.
  • 细胞定位Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt

Recombinant Human alpha A Crystallin protein 图像

  • SDS-PAGE showing ab113189
    Lane 1: 0.5µg protein
    Lane 2: 1.0µg protein
    Lane 3: 2.0µg protein
    Lane 4: 4.0µg protein
  • All lanes : anti-alpha A Crystallin pAb

    Lane 1 : Recombinant Human alpha A Crystallin protein (ab113189) at 0.1 µg
    Lane 2 : Recombinant Human alpha A Crystallin protein (ab113189) at 0.2 µg
    Lane 3 : Native Cow alpha A Crystallin protein

Recombinant Human alpha A Crystallin protein (ab113189)参考文献

ab113189 has not yet been referenced specifically in any publications.

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