重组人CTNNA1蛋白(Tagged) (ab51443)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: = 95% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB
描述
-
产品名称
重组人CTNNA1蛋白(Tagged) -
纯度
= 95 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
-
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
预测分子量
123 kDa including tags -
标签
GST tag N-Terminus -
额外的序列信息
NM 001903
-
技术指标
Our Abpromise guarantee covers the use of ab51443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
SDS-PAGE
Western blot
-
形式
Liquid -
补充说明
This product was previously labelled as alpha 1 Catenin
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
常规信息
-
别名
- Alpha E-catenin
- Cadherin associated protein
- Cadherin associated protein 102kDa
see all -
功能
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. -
组织特异性
Expressed ubiquitously in normal tissues. -
序列相似性
Belongs to the vinculin/alpha-catenin family. -
翻译后修饰
Sumoylated. -
细胞定位
Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab51443 被引用在 1 文献中.
- Yosefzon Y et al. Caspase-3 Regulates YAP-Dependent Cell Proliferation and Organ Size. Mol Cell 70:573-587.e4 (2018). Functional Studies . PubMed: 29775577