Recombinant人ALDH2 protein (ab87415)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S

技术指标

Our Abpromise guarantee covers the use of ab87415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity is < 0.2 units/ml, and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 umole of acetaldehyde to acetic acid per minute at pH 8.0 at 25C in the presence of beta-NAD, potassium and thiols.
  • 应用

    SDS-PAGE

    Functional Studies

    Western blot

  • 纯度> 90 % SDS-PAGE.
    ab87415 is purified using conventional chromatography techniques.
  • 形式Liquid
  • 补充说明Endotoxin:< 1.0 EU per 1 µg of protein (determined by LAL method).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Acetaldehyde dehydrogenase 2
    • Aldehyde dehydrogenase 2 family
    • Aldehyde dehydrogenase 2 family (mitochondrial)
    • Aldehyde dehydrogenase mitochondrial
    • Aldehyde dehydrogenase, mitochondrial
    • ALDH 2
    • ALDH class 2
    • ALDH E2
    • ALDH-E2
    • Aldh2
    • ALDH2_HUMAN
    • ALDHI
    • ALDM
    • Liver mitochondrial ALDH
    • MGC1806
    • Mitochondrial aldehyde dehydrogenase 2
    • MS767
    • Nucleus encoded mitochondrial aldehyde dehydrogenase 2
    see all
  • 通路Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
  • 序列相似性Belongs to the aldehyde dehydrogenase family.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt

Recombinant human ALDH2 protein 图像

  • 15% SDS-PAGE showing 3µg ab87415 at approximately 54.5 kDa.
  • All lanes : Anti-ALDH2 antibody (ab70917) at 1/100 dilution

    Lane 1 : Natural human Alpha 2 Antiplasmin protein (ab77936) at 0.1 µg
    Lane 2 : Natural human Alpha 2 Antiplasmin protein (ab77936) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

Recombinant human ALDH2 protein (ab87415)参考文献

ab87415 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. The specific information activity for these products is as follows:


ab87415: Specific activity is > 0.14 units/ml

ab93463 and ab117154: Specific activity is > 0.5 units/ml

The protocol used...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"