Recombinant人AlaRS protein (ab73442)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 分子量108 kDa
    • 额外的序列信息Alanyl-tRNA synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 107,598 Dalton

技术指标

Our Abpromise guarantee covers the use of ab73442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性ab73442 is reactive against Polymyositis sera.
  • 应用

    Western blot

    ELISA

    SDS-PAGE

  • 纯度> 90 % SDS-PAGE.
    ab73442 is purified by proprietary chromatographic techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 250mM Sodium chloride, 16mM HEPES, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • AARS
    • AI316495
    • Alanine tRNA ligase
    • Alanine tRNA ligase 1, cytoplasmic
    • Alanine tRNA ligase cytoplasmic
    • Alanine--tRNA ligase
    • Alanyl tRNA synthetase
    • Alanyl tRNA synthetase cytoplasmic
    • Alanyl-tRNA synthetase
    • AlaRS
    • C76919
    • CMT2N
    • cytoplasmic
    • EC 6.1.1.7
    • MGC37368
    • Renal carcinoma antigen NY REN 42
    • Renal carcinoma antigen NY-REN-42
    • SYAC_HUMAN
    see all
  • 功能Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
  • 疾病相关Charcot-Marie-Tooth disease 2N
  • 序列相似性Belongs to the class-II aminoacyl-tRNA synthetase family.
  • 结构域Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
    The C-terminal C-Ala domain (residues 756 to 968), along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. The human domain can be used in vitro to replace the corresponding domain in E.coli.
  • 翻译后修饰ISGylated.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant human AlaRS protein (ab73442)参考文献

ab73442 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab73442.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"