Recombinant人AKT2 protein (ab79798)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionP31751
    • 种属Human
    • 序列MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLP PLNNFSVAEC QLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREE WMRAIQMVANSLKQRAPGEDPM DYKCGSPSDSSTTEEMEVAVSKARAK VTMNDFDYLKLLGKGTFGKVILVREKATGRYYAM KILRKEVIIAKDEV AHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFH LS RERVFTEERARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKIT DFGLCKEG ISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVM YEMMCGRLPFYNQDHERLFE LILMEEIRFPRTLSPEAKSLLAGLLKKD PKQRLGGGPSDAKEVMEHRFFLSINWQDVVQK KLLPPFKPQVTSEVDT RYFDDEFTAQSITITPPDRYDSLGLLELDQRTHFPQFSYSASIR E
    • 氨基酸1 to 481
    • 标签His tag N-Terminus
    • 额外的序列信息Recombinant full-length human AKT2 was co-expressed with PDK1 by baculovirus infection in Sf9 insect cells.

技术指标

Our Abpromise guarantee covers the use of ab79798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific Activity: 5.9 pmol/min/µg.
  • 应用

    Functional Studies

    SDS-PAGE

    Western blot

  • 纯度>90% by SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 0.1% Imidazole
    Constituents: 0.0462% DTT, 0.395% Tris HCl, 50% Glycerol, 0.58% Sodium chloride, Potassium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Akt2
    • AKT2_HUMAN
    • HIHGHH
    • murine thymoma viral (v-akt) homolog-2
    • PKB
    • PKB beta
    • PKBB
    • PKBBETA
    • PRKBB
    • Protein kinase Akt 2
    • Protein kinase Akt-2
    • Protein kinase B beta
    • rac protein kinase beta
    • RAC-BETA
    • RAC-beta serine/threonine-protein kinase
    • RAC-PK-beta
    • v akt murine thymoma viral oncogene homolog 2
    see all
  • 功能General protein kinase capable of phosphorylating several known proteins.
  • 组织特异性Expressed in all human cell types so far analyzed.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylation on Thr-309 and Ser-474 is required for full activity.
    Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Information by UniProt

Recombinant human AKT2 protein 图像

  • Anti-AKT2 antibody (ab78666) + Recombinant human AKT2 protein (ab79798) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
  • 10% SDS-PAGE showing ab79798 at approximately 57kDa (10µg).

  • Kinase assay: Specific activity 5.9 pmol/min/μg.

Recombinant human AKT2 protein (ab79798)参考文献

ab79798 has not yet been referenced specifically in any publications.

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