The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific activity: approximately 0.15 - 0.2 units/mg.
Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C. Specific activity was expressed as units/mg protein.
% SDS-PAGE. ab117472 is purified by proprietary chromatographic techniques.
ab117472 is stable at 4°C for 30 days, it should be stored desiccated below -20°C for periods greater than 30 days. Avoid freeze-thaw cycles.
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Shipped at 4°C. Please see notes section.
pH: 8.00 Constituents: 0.32% Tris HCl, 0.02% DTT, 20% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation.
Expressed in all tissues tested including liver, prostate, testis, adrenal gland, brain, uterus, mammary gland and keratinocytes. Highest levels found in liver, mammary gland and brain.