Recombinant人AKR1B1 protein (ab117190)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP15121
    • 种属Human
    • 序列MASRLLLNNGAKMPILGLGTWKSPPGQVTEAVKVAIDVGYRHIDCAHVYQ NENEVGVAIQEKLREQVVKREELFIVSKLWCTYHEKGLVKGACQKTLSDL KLDYLDLYLIHWPTGFKPGKEFFPLDESGNVVPSDTNILDTWAAMEELVD EGLVKAIGISNFNHLQVEMILNKPGLKYKPAVNQIECHPYLTQEKLIQYC QSKGIVVTAYSPLGSPDRPWAKPEDPSLLEDPRIKAIAAKHNKTTAQVLI RFPMQRNLVVIPKSVTPERIAENFKVFDFELSSQDMTTLLSYNRNWRVCA LLSCTSHKDYPFHEEF
    • 分子量36 kDa
    • 氨基酸1 to 316

技术指标

Our Abpromise guarantee covers the use of ab117190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity : approximately 0.5 - 0.6 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C. Specific activity was expressed as units/mg protein.
  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 95 % SDS-PAGE.
    ab117190 is purified by proprietary chromatographic techniques.
  • 形式Liquid
  • 补充说明Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Please see notes section.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 0.02% DTT, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • ADR
    • AKR1B 1
    • Akr1b1
    • Aldehyde reductase
    • Aldehyde reductase 1
    • Aldo keto reductase family 1, member B1
    • Aldo-keto reductase family 1 member B1
    • aldo-keto reductase family 1, member B1 (aldose reductase)
    • Aldose reductase
    • aldr 1
    • ALDR_HUMAN
    • aldr1
    • ALR2
    • AR
    • Lii5 2 CTCL tumor antigen
    • Low Km aldose reductase
    • MGC1804
    see all
  • 功能Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
  • 组织特异性Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.
  • 序列相似性Belongs to the aldo/keto reductase family.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant human AKR1B1 protein (ab117190)参考文献

ab117190 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

I spoke to the lab again and this time they were able to supply the raw data that you were asking for. This information is in the Excel file that is attached.

If there is anything else I can help you with, pl...

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Thank you for contacting Abcam.

I have talked to the lab and we are unable to send you the actual raw QC data, but below is the protocol they used to test the activity of ab117190:

Activity Assay:

1. Prepare a 1ml reaction mi...

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Thank you for contacting us today and your interest in our products.

I can now confirm that the specific activity figures I quoted to you over the phone did relate to the most current lot which you have received. Enzymatic activity was confir...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"