Recombinant人ADRB2 + GsaL-Leu227 fusion protein (ab90834)

概述

  • 产品名称Recombinant人ADRB2 + GsaL-Leu227 fusion protein
  • 蛋白长度Full length protein
  • 描述
    Recombinant人ADRB2 + GsαL-Leu227 fusion protein

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 分子量104 kDa
    • 额外的序列信息Tagged at the N terminus of the ADRB2 with a DDDDK tag. The C terminus of the ADRB2 is linked to the N terminus of the Gs alpha L with a His tag.

技术指标

Our Abpromise guarantee covers the use of ab90834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Activity: 7 pmol/mg.
  • 应用

    Functional Studies

  • 形式Liquid
  • 补充说明

    The exchange of Gln227 to Leu227 (Q/L mutation) in the catalytic site abolishes the intrinsic GTPase activity and increases the GDP-affinity of GSalpha, resulting in a constitutively active G-protein.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 75mM Tris HCl, 12.5mM Magnesium chloride, 1mM EDTA, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Adenylate cyclase stimulating G alpha
    • ADRB 2
    • ADRB2
    • ADRB2R
    • ADRBR
    • Adrenergic beta 2 receptor surface
    • B2AR
    • BAR
    • Beta 2 adrenoceptor
    • Beta 2 adrenoreceptor
    • BETA2AR
    • Catecholamine receptor
    • GNAS
    • GNAS 2
    • GNAS complex locus
    • GSP
    • Guanine nucleotide binding protein G(s) subunit alpha
    see all
  • 相关性ADBR2 is a member of the G protein-coupled receptor superfamily. It is activated by the catecholamine epinephrine and couples to the G protein Gs to mediate adenylate cyclase activation. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli.
  • 细胞定位ADBR2: Cell Membrane

Recombinant human ADRB2 + GsaL-Leu227 fusion protein (ab90834)参考文献

ab90834 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab90834.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"