Recombinant人Activin A protein (ab50051)

概述

描述

  • 性质Recombinant
  • 来源BTI-TN-5B1-4 cells
  • 氨基酸序列
    • AccessionP08476
    • 种属Human
    • 序列GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS
    • 氨基酸311 to 426
    • 额外的序列信息The sequence is the full length mature protein minus signal peptide & propeptide. This protein is Activin A, 26.0 kDa disulfide-linked homodimer of 2 βA chains, each containing 116 aa residues.

相关产品

技术指标

Our Abpromise guarantee covers the use of ab50051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    Biological Activity : The ED50 as determined by its ability to inhibit the proliferation of murine MPC-11 cells is < 2 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.

  • 应用

    Cell differentiation

    SDS-PAGE

    Functional Studies

  • 纯度> 95 % SDS-PAGE.
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • 形式Lyophilised
  • 补充说明This protein is expressed in (BTI-Tn-5B1-4) High-5 Insect Cells
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    pH: 3.00
    Constituent: 0.2941% Sodium citrate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.

常规信息

  • 别名
    • Activin A
    • Activin beta A chain
    • Activin beta-A chain
    • ActivinA
    • EDF
    • Erythroid differentiation factor
    • Erythroid differentiation protein
    • INHBA
    • INHBA_HUMAN
    • Inhibin beta A chain
    see all
  • 功能Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • 序列相似性Belongs to the TGF-beta family.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant human Activin A protein 图像

  • Human embryonic stem cells (HUES4) were differentiated as monolayer cultures on matrigel coated dishes using a protocol described in Kroon et al, Nat Biotechnol. (2008). Activin A (ab50051) and Wnt3a (ab81484) were added to cultures to direct differentiation towards definitive endoderm. This image was taken 3 days after induction, where the definitive endoderm marker FOXA2 (ab40874 in green) is abundant. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.

Recombinant human Activin A protein (ab50051)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Te pido disculpas por la espera.

He comprobado con mis compañeros del laboratorio que ninguna de las proteínas Inhibina A o B disponibles actualmente en el catalogo corresponde al heterodímero activo.

Sin embarg...

Read More

Thank you for contacting us. The expected molecular weight for Inhibin beta A protein ab50051 is 26.0kDa. Please be advised that ab25747 is not a full length protein it is a peptide which we have used to produce antibody ab24562. Could you p...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"