Recombinant人active Caspase-3 protein (ab52101)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列

技术指标

Our Abpromise guarantee covers the use of ab52101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    SPECIFIC ACTIVITY: 300,000-400,000 units/mg UNIT DEFINITION: One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

  • 应用

    Functional Studies

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • 补充说明The lyophilized caspase-3 is stable for 1 year at –70oC The recombinant active human Caspase 3 spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme.


    Active Caspase 3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. The active Caspase 3 preferentially cleaves Caspase 3 substrates e.g. DEVD-AFC or DEVD-pNA.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 15% Glycerol, PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute to 1 unit per µl in PBS containing 15% glycerol.

常规信息

  • 别名
    • Apopain
    • CASP 3
    • CASP-3
    • CASP3
    • CASP3_HUMAN
    • Caspase 3
    • Caspase-3 subunit p12
    • CPP 32
    • CPP-32
    • CPP32B
    • Cysteine protease CPP32
    • PARP cleavage protease
    • Protein Yama
    • SCA-1
    • SCA1
    • SREBP cleavage activity 1
    • Yama
    see all
  • 功能Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
  • 组织特异性Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • 序列相似性Belongs to the peptidase C14A family.
  • 翻译后修饰Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant human active Caspase-3 protein (ab52101)参考文献

ab52101 has not yet been referenced specifically in any publications.

Product Wall

The active Caspase 3 protein (Active), ab52101, is provided at the equivalent of 100 units. There are between 300,000-400,000 units/mg. Therefore a total of between 0.25-0.33 ug of protein is provide per vial.

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from our Anti-Caspase 3 antibody [4H334] (ab17819) and active Caspase 3 protein (Active) (ab52101) . <...

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Thank you again for your call.

Unfortunately, the total concentration of this protein is proprietary information. We sell these by the number of units of enzyme activity totally present in each vial. Therefore in case of ab52101, there would ...

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Thank you for contacting us.

I would suggest to use ab17819 Anti-Caspase 3 antibody [4H334]

http://www.abcam.com/index.html?datasheet=17819 (or use the following: http://www.abcam.com/index.html?datasheet=17819).

Even if ...

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Thank you for providing that information and sorry for the delay in getting back to you.
The information provided has been very useful and I will look into whether these antibodies would be likely to detect the protein and what may be contributing...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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You have received the recombinant full length protein: processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme.

Thank you for calling Abcam earlier today.

I am sorry about the issues you have been having with the Caspase 3 protein. As we discussed, I have requested that you be sent (via Cedarlane) a free of charge replacement for the protein.

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Thank you for contacting Abcam.

I have emailed the lab about your enquiry and below is their response:

This product is not sold by concentration. It is sold by Units. The recombinant active human caspase-3 expressed in E coli sponta...

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Thank you for contacting Abcam.

For ab39901, Caspase 1, we recommend using 1 unit/assay for analyzing caspase activity and this protein has a biological activity : >5000 units/mg and it is supplied lyophilized and it should be reconstitute...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"