The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SPECIFIC ACTIVITY: 10,000 – 13,000 units/mg
UNIT DEFINATION: One unit of the recombinant caspase-2 is the enzyme activity that cleaves 1 nmol of the caspase substrate VDVAD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT
The lyophilized caspase-2 is stable for 1 year at –70oC.
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Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
NEDD 2 apoptosis regulatory gene
Caspase 2 is involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. During apoptosis, procaspase-2 is processed at aspartate residues. The processed form of caspase-2 consists of large (19 kDa) and small (12 kDa) subunits which associate to form the active enzyme.
Recombinant human active Caspase 2 protein (ab52098)参考文献
has not yet been referenced specifically in any publications.