重组人14-3-3 zeta蛋白(ab85268)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, WB
描述
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产品名称
重组人14-3-3 zeta蛋白
参阅全部 14-3-3 zeta 蛋白酶 -
纯度
> 95 % SDS-PAGE.
Purity was determined to be >95% by densitometry. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab85268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
常规信息
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别名
- 14 3 3 delta
- 14 3 3 protein zeta/delta
- 14 3 3 protein/cytosolic phospholipase A2
see all -
功能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
序列相似性
Belongs to the 14-3-3 family. -
翻译后修饰
The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1. -
细胞定位
Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab85268 被引用在 1 文献中.
- Liu QW et al. N-linked glycoproteomic profiling in esophageal squamous cell carcinoma. World J Gastroenterol 28:3869-3885 (2022). PubMed: 36157541