Recombinant人14-3-3 zeta protein (ab116445)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP63104
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMDKNELVQKAKLAEQA ERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIE QKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKV FYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPI RLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTL IMQLLRDNLTLWTSDTQGDEAEAGEGGEN
    • 分子量31 kDa including tags
    • 氨基酸1 to 245
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab116445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF-TOF
  • 纯度> 80 % Purified via His tag.

  • 形式Liquid
  • 补充说明ab116445 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF. The peptides obtained gave 77 % sequence coverage of 14-3-3 zeta. Theoretical pI: 5.23
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.6% HEPES, 50% Glycerol, 0.02% DTT, 0.003% PMSF, 0.012% Benzamidine

常规信息

  • 别名
    • 14 3 3 delta
    • 14 3 3 protein zeta/delta
    • 14 3 3 protein/cytosolic phospholipase A2
    • 14 3 3 zeta
    • 14-3-3 protein zeta/delta
    • 1433Z_HUMAN
    • Epididymis luminal protein 4
    • Epididymis secretory protein Li 3
    • HEL S 3
    • HEL4
    • KCIP-1
    • KCIP1
    • MGC111427
    • MGC126532
    • MGC138156
    • Phospholipase A2
    • Protein kinase C inhibitor protein 1
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide
    • YWHAD
    • YWHAZ
    see all
  • 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • 序列相似性Belongs to the 14-3-3 family.
  • 翻译后修饰The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
  • 细胞定位Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
  • Information by UniProt

Recombinant Human 14-3-3 zeta protein 图像

  • 12% SDS-PAGE gel showing ab116445.

Recombinant Human 14-3-3 zeta protein (ab116445)参考文献

ab116445 has not yet been referenced specifically in any publications.

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