The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% Purified via His tag. ab116444 was purified via Ni2+-NTA agarose columns.
ab116444 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF mass spectrometry. The peptides obtained gave 64 % sequence coverage of 14-3-3 theta.
Theoretical pI: 5.2
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Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
Belongs to the 14-3-3 family.
Ser-232 is probably phosphorylated by CK1.
Cytoplasm. In neurons, axonally transported to the nerve terminals.