The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% Purified via His tag. ab116444 was purified via Ni2+-NTA agarose columns.
补充说明ab116444 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF mass spectrometry. The peptides obtained gave 64 % sequence coverage of 14-3-3 theta.
Theoretical pI: 5.2
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Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
组织特异性Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
序列相似性Belongs to the 14-3-3 family.
翻译后修饰Ser-232 is probably phosphorylated by CK1.
细胞定位Cytoplasm. In neurons, axonally transported to the nerve terminals.