Recombinant人14-3-3 Tau protein (ab116444)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP27348
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMEKTELIQKAKLAEQA ERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIE QKTDTSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKV FYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPI RLGLALNFSVFYYEILNNPELACTLAKTAFDEAIAELDTLNEDSYKDSTL IMQLLRDNLTLWTSDSAGEECDAAEGAEN
    • 分子量31 kDa including tags
    • 氨基酸1 to 245
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab116444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Mass Spectrometry

    SDS-PAGE

  • 质谱法
    MALDI-TOF-TOF
  • 纯度> 80 % Purified via His tag.
    ab116444 was purified via Ni2+-NTA agarose columns.
  • 形式Liquid
  • 补充说明ab116444 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF mass spectrometry. The peptides obtained gave 64 % sequence coverage of 14-3-3 theta. Theoretical pI: 5.2
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.6% HEPES, 0.02% DTT, 50% Glycerol, 0.004% PMSF, 0.012% Benzamidine

常规信息

  • 别名
    • 14 3 3 protein T cell
    • 14 3 3 protein tau
    • 14 3 3 protein theta
    • 14 3 3 tau
    • 14 3 3 theta
    • 14-3-3 protein T-cell
    • 14-3-3 protein tau
    • 14-3-3 protein theta
    • 1433T_HUMAN
    • 1C5
    • HS1
    • KCIP1
    • Protein HS1
    • Protein kinase C inhibitor protein 1
    • Protein tau
    • tyr3/trp5 monooxygenase activation protein, theta
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, theta polypeptide
    • Ywhaq
    see all
  • 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
  • 组织特异性Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
  • 序列相似性Belongs to the 14-3-3 family.
  • 翻译后修饰Ser-232 is probably phosphorylated by CK1.
  • 细胞定位Cytoplasm. In neurons, axonally transported to the nerve terminals.
  • Information by UniProt

Recombinant Human 14-3-3 Tau protein 图像

  • 12% SDS-PAGE gel showing ab116444.

Recombinant Human 14-3-3 Tau protein (ab116444)参考文献

ab116444 has not yet been referenced specifically in any publications.

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