Recombinant人14-3-3 eta protein (ab116442)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ04917
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMGDREQLLQRARLAEQ AERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSI EQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFLIKNCNDFQ YESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQ PTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSY KDSTLIMQLLRDNLTLWTSDQQDEEAGEGN
    • 分子量32 kDa including tags
    • 氨基酸1 to 246
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab116442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF-TOF
  • 纯度> 80 % Purified via His tag.
    ab116442 was purified by Ni2+-NTA agarose
  • 形式Liquid
  • 补充说明ab116442 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF. The peptides obtained gave 67% sequence coverage of 14-3-3 eta. Theoretical pI: 5.3
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.6% HEPES, 0.02% DTT, 50% Glycerol, 0.012% Benzamidine, 0.003% PMSF

常规信息

  • 别名
    • 14 3 3 protein eta
    • 14-3-3 eta
    • 14-3-3 protein eta
    • 1433F_HUMAN
    • Brain protein 14-3-3, eta isoform
    • HGNC:12853
    • Protein AS1
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform
    • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide
    • YWHA 1
    • YWHA1
    • Ywhah
    see all
  • 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • 组织特异性Expressed mainly in the brain and present in other tissues albeit at lower levels.
  • 序列相似性Belongs to the 14-3-3 family.
  • Information by UniProt

Recombinant Human 14-3-3 eta protein 图像

  • 12 % SDS-PAGE gel showing ab116442.

Recombinant Human 14-3-3 eta protein (ab116442)参考文献

ab116442 has not yet been referenced specifically in any publications.

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