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Our Abpromise guarantee covers the use of ab84117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
One unit of HIV Protease hydrolyzes 1 picomole of a peptide (SQNYPIVQ) per minute at pH 4.7 at 25oC. 20-200ng is sufficient for an in vitro protease assay. HIV Protease can be applied in in vitro assay development and screening of protease inhibitors.
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 20% Glycerol, 20mM Tris HCl, 500mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
This product is an active protein and may elicit a biological response in vivo, handle with caution.