重组HIV1 p55蛋白(ab63995)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE
描述
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产品名称
重组HIV1 p55蛋白 -
纯度
> 90 % SDS-PAGE.
Purified by several steps of chromatography -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
技术指标
Our Abpromise guarantee covers the use of ab63995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
补充说明
ab63995 has been derived from infectious plasmid clone pNL4-3 and belongs to Group B of HIV-1.
Active as HIV-1 protease substrate
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 6
Constituents: 0.078% Beta mercaptoethanol, 0.316% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
常规信息
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别名
- gag
- Gag polyprotein
- Pr55Gag
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相关性
HIV1 (gag) p55 is a precursor protein of several proteins that form the core structure of AIDS virus, indispensable to its reproduction. This protein is digested by HIV1 protease, first into intermediate products p41 and p15. Then p41 is digested into matrix protein p17 and capsid protein p24. Protein p15 is further digested into nucleocapsid protein p7 and to p6 and p1; of unknown function. Nine genes are encoded within the HIV1 RNA genome. Three of these genes, gag, pol, and env, contain information needed to make the structural proteins for new virus particles.
图片
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SDS-PAGE analysis of Recombinant HIV1 p55 protein. As the substrate, Recombinant HIV1 p55 protein (1 μg, ab63995) was used in 20 μl reaction volume. The reaction was carried by incubating at 37℃ for 3 h and stopped by adding SDS-PAGE sample buffer. Lane 1: no protease,
Lane 2: 0.16 pg.
Lane 3: 1.6 pg.
Lane 4: 16 pg
Lane 5: 0.16 μg
Lane 6: 1.6 μg protease.
Note that two degradation bands are observed in the preparation of p55 substrate. In lane 4, p25 band is visible and in lane 5, p13 band is visible. -
Polyacrylamide gel electrophoresis of ab63995.
The arrows show degradation products.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab63995 尚未被引用在任何文献中。