Recombinant丙型肝炎病毒NS4 protein (FITC) (ab49029)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 氨基酸1658 to 1863
  • 偶联物FITC. Ex: 493nm, Em: 528nm

技术指标

Our Abpromise guarantee covers the use of ab49029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Reacts strongly with human HCV positive serum.
  • 应用

    Western blot

    ELISA

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • 补充说明Reacts strongly with human HCV positive serum.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 8M Urea, 20mM Tris HCl, 10mM Beta mercaptoethanol, pH 8.0

常规信息

  • 别名
    • HCV
    • HCV NS4
    • Hepatitis C Virus nonstructural antigen 4
    • Non structural protein 4A
    • Non structural protein 4B
    • NS4A
    • NS4B
    • p27
    • p8
    see all
  • 相关性NS4 A/B are two of the seven nonstructural (NS) proteins making up the HCV polyprotein. The NS proteins recruit the viral genome into an RNA replication complex, which is associated with rearranged cytoplasmic membranes. NS4A acts as a cofactor with the NS3 serine protease and stabilizes its folding. The NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3. HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. HCV is responsible for a large proportion of worldwide chronic viral hepatitides. Most of these infections develop into chronic hepatitis, which often progresses to liver cirrhosis and hepatocellular carcinoma. At present, (unlike hepatitis A and B), there is no vaccine to prevent hepatitis C infection. The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is a relatively hydrophobic 27-kDa protein. The 4A protein has a molecular weight of 6 kDa.
  • 细胞定位Endoplasmic reticulum; multi-pass membrane protein.

Recombinant Hepatitis C Virus NS4 protein (FITC) (ab49029)参考文献

ab49029 has not yet been referenced specifically in any publications.

Product Wall


The ELISA protocol is below.
If antigen is diluted at least 1 to 10 the 8M Urea will not be a problem.


ELISA protocol

1. Dilute HCV antigen to 0.1 mg/25 ml (4.0 mg/ml) in coating buffer (0.01M Na2CO3; 0.01M Na3EDTA...

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The lab let me know that they used a standard ELISA protocol with 50 microgram per well as a starting concentration with serial dilutions. The urea did not interfere.

Thank you for contacting us. In theory the protein ab49029 should react with antibidy ab124526, since the protein is full length Hepatitis C Virus NS4 protein. However, this has not been tested and there is a possibility the tag could prevent the antib...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"