Recombinant丙型肝炎病毒NS3 protein (Biotin) (ab68047)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 氨基酸
      1450 to 1643
    • 额外的序列信息
      The E.coli derived recombinant Biotin Labeled protein contains the HCV NS3 immunodominant regions. The Biotin labeled protein is fused with b-galactosidase (114 kDa) at N-terminus.
  • 偶联物
    Biotin

技术指标

Our Abpromise guarantee covers the use of ab68047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    ab68047 is immunoreactive with sera of HCV-infected individuals.
  • 应用

    Western blot

    ELISA

  • 纯度
    > 95 % SDS-PAGE.
    ab68047 was purified by a proprietary chromatographic technique.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 1.5M Urea, 25mM Tris HCl, 1mM EDTA, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Hepatitis C Virus NS3
    • NS3
    • NS3P
    • p70
    • Serine protease/NTPase/helicase
    see all
  • 相关性
    HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • 细胞定位
    Endoplasmic reticulum membrane

文献

ab68047 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us.

Please accept my apologies for the delay, it was a very comprehensive search.

Unfortunately, I have to let you know that we do not have a suitable HCV NS3 protein available that can be used as a positive...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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