Recombinant丙型肝炎病毒Genotype 1a NS3 protein (ab123483)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP27958
    • 分子量30 kDa
    • 氨基酸1 to 271
    • 标签His-DDDDK tag N-Terminus
    • 额外的序列信息The NS4A co-factor is fused to the N-terminus of the NS3 protease domain.

技术指标

Our Abpromise guarantee covers the use of ab123483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性One unit of protease hydrolyzes 1 picomole of Ac-Asp-Glu-Dap(QXL™520)- Glu-Glu-Abu-COO-Ala -Ser-Cys(5-FAMsp)-NH2) per minute at pH 7.5 at 25° C.
    ab123483 is in active form and the pre-activation by pep4A or pep4AK is not necessary.
  • 应用

    Functional Studies

  • 形式Liquid
  • 补充说明


    NS3 protease of hepatitis C virus (HCV), located on the N-terminal domain of HCV NS3, is responsible for the cleavage at the NS3/NS4A, NS4A/NS4B, NS4B/NS5A, and NS5A/NS5B sites of the nonstructural protein. The HCV NS3 is a chymotrypsin-like serine protease. It requires a cofactor, a 54 amino acid NS4 protein, to reach its optimal activity. The X-ray crystal structure studies show that NS3 forms a tight non-covalent complex with NS4. The NS3/4A protease is essential for viral replication and the formation of infectious viral particles.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.75% Potassium chloride, 0.003% DTT, 0.32% Tris HCl, 0.06% EDTA, 20% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Serine protease/NTPase/helicase NS3
  • 相关性The nonstructural protein NS3 of the hepatitis C virus (HCV) is indispensable for virus replication and a multifunctional enzyme that contains three catalytic activities such as serine protease, helicase, and NTPase. The N-terminal domain of the protein contains protease activity and the C-terminal domain contains nucleotide triphosphatase and RNA helicase activity. It has been shown that NS2/3 cleavage is mediated by NS2-3 protease, whereas NS3 serine protease is responsible for the other four cleavage sites of the nonstructural (NS) region. Immunoblot analysis on serum samples from 90 patients with chronic hepatitis C virus infection revealed four putative immunogenic regions within the NS3 protein of the virus: E (around amino acids 1250/1251), A (within amino acids 1250-1334), A/B (around amino acids 1323 and 1334), and B/C (around amino acids 1407 and 1412). Among them, region E was most immunodominant, and region A was recognized much less frequently by patients with cirrhosis than by those with chronic hepatitis.
  • 细胞定位Host endoplasmic reticulum membrane; Peripheral membrane protein. Note: NS3 is associated to the ER membrane through its binding to NS4A.

Recombinant Hepatitis C virus Genotype 1a NS3 protein (ab123483)参考文献

ab123483 has not yet been referenced specifically in any publications.

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