Recombinant Hepatitis B Surface Antigen (Adw) protein (ab91276)

概述

描述

  • 性质Recombinant
  • 来源Pichia pastoris

技术指标

Our Abpromise guarantee covers the use of ab91276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    ELISA

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    ab91276 is purified by proprietary chromatographic techniques. Purity is >95% by SDS-PAGE and RP-HPLC.
  • 形式Liquid
  • 补充说明


    ab91276 contains the Hepatitis B Virus Surface Antigen immunodominant region.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at 4°C or -20°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20mM PBS, 154mM Sodium chloride, pH 7.2

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • HBsAg
    • S gene subtype adw2
    • sAg
    • ssAg
  • 相关性Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
  • 细胞定位Membrane Virion

Recombinant Hepatitis B Surface Antigen (Adw) protein (ab91276)参考文献

ab91276 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

This product is not tesed for endotoxins.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

We have not conducted any research experiements with this recombinant protein beyond noting that ab91276 is immunoreactive with sera of Hepatitis B virus-infected individuals.

The 226 amino acid protein is based on the sequence at the followi...

Read More

Thank you for contacting Abcam.

We have a number of antibodies against Hepatitis B Surface Antigen and I have placed links to them below:

http://www.abcam.com/Hepatitis-B-Surface-Antigen-antibody-1044329-ab128198.html
http://ww...

Read More

Thank you for taking the time to complete the questionnaire and send an image. This has been helpful to our quality investigations.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the re...

Read More

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I have discussed this with our testing source and can confrim that this antibody should only detect th...

Read More

Merci de nous avoir contactés.

ab67704 : tag GST, 40 kDa
ab69741 : pas de tag, 57.5 kDa
ab69748 : pas de tag, 64.5 kDa
ab69749 : pas de tag, 70 kDa
ab91276 : pas de tag, 24 kDa

J'espère que ces info...

Read More
Application Other

Review text: Used as a standardised positive control for an in-house HBsAg ELISA as well as commercial ELISA. Used at concentrations ranging from 10ng to 0.125ng - all concentrations gave positive results on ELISAs.
Sample: Human Serum

Primary antibody (in addition to 'Hepatitis B Surface Antigen (Adw) protein')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used
Username

Abcam user community

Verified customer

提交于 Dec 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"