Recombinant HDAC3+NCOR2 complex protein (ab42631)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus

技术指标

Our Abpromise guarantee covers the use of ab42631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Activity: 110 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM Biomol substrate (Catalog number KI177), and 1 ng/ul HDAC3/NcoR2. Incubation condition: 30 min at 37ºC.
  • 应用

    Inhibition Assay

  • 纯度> 90 % SDS-PAGE.

  • 形式Liquid
  • 补充说明Co-expressed in a Baculovirus infected Sf9 cell expression system.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 50mM Tris HCl, 20mM Glutathione, 138mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • CTG26
    • HD 3
    • HD3
    • HDAC 3
    • HDAC3
    • HDAC3_HUMAN
    • Histone deacetylase 3
    • NCOR 2
    • Nuclear receptor co repressor 2
    • RPD3
    • RPD3-2
    • SMAP45
    • SMRTE
    • SMRTE tau
    • TNRC14
    • TRAC1
    see all
  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻译后修饰Sumoylated in vitro.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant HDAC3+NCOR2 complex protein (ab42631)参考文献

This product has been referenced in:
  • Demmerle J  et al. The nuclear envelope protein emerin binds directly to histone deacetylase 3 (HDAC3) and activates HDAC3 activity. J Biol Chem : (2012). Read more (PubMed: 22570481) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"