Recombinant E. coli Thioredoxin / TRX protein (ab115709)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP0AA25
    • 种属Escherichia coli
    • 分子量12 kDa
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab115709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Western blot

    Functional Studies

  • 纯度> 95 % SDS-PAGE.
    ab115709 is purified by multi-step chromatography
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.61% Tris, 0.58% Sodium chloride, 0.03% EDTA

常规信息

  • 别名
    • ADF
    • ATL derived factor
    • ATL-derived factor
    • DKFZp686B1993
    • MGC61975
    • SASP
    • Surface associated sulphydryl protein
    • Surface-associated sulphydryl protein
    • testicular tissue protein Li 199
    • THIO_HUMAN
    • Thioredoxin
    • thioredoxin delta 3
    • TRDX
    • Trx
    • TRX 1
    • TRX1
    • TXN
    • TXN delta 3
    • TXN protein
    • zgc:92903
    see all
  • 功能Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
    ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
  • 序列相似性Belongs to the thioredoxin family.
    Contains 1 thioredoxin domain.
  • 翻译后修饰In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
    In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
  • 细胞定位Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
  • Information by UniProt

Recombinant E. coli Thioredoxin / TRX protein 图像

  • Thioredoxin /TRX antibody + Recombinant E. coli Thioredoxin / TRX protein (ab115709) at 0.1 µg
  • SDS-PAGE analysis
    Lane 1: MW markers
    Lane 2: ab115709 at 1 µg
    Lane 3: ab115709 at 2 µg
    Lane 4: ab115709 at 5 µg

Recombinant E. coli Thioredoxin / TRX protein (ab115709)参考文献

ab115709 has not yet been referenced specifically in any publications.

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