The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab117663 is purified by proprietary chromatographic techniques. Purity is greater than 85.0% as determined by SDS-PAGE.
Although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
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Shipped at 4°C. Please see notes section.
pH: 8.00 Constituents: 0.32% Tris HCl, 10% Glycerol, 0.02% DTT
Surface associated sulphydryl protein
Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/ dithiol group within the conserved Cys-Gly-Pro-Cys active site. Specific protein disulfide targets for reduction by Trx include protein disulfide –isomerase (PDI) and a number of transcription factors such as p53, NFkB and AP-1. Trx is also apable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.
Recombinant E. coli Thioredoxin 1 protein (ab117663)参考文献
has not yet been referenced specifically in any publications.