Recombinant E. coli surA protein (ab99241)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP0ABZ6
    • 种属Escherichia coli
    • 序列MGSSHHHHHH SSGLVPRGSH M APQVVDKVA AVVNNGVVLE SDVDGLMQSV KLNAAQARQQ LPDDATLRHQ IMERLIMDQI ILQMGQKMGV KISDEQLDQA IANIAKQNNM TLDQMRSRLA YDGLNYNTYR NQIRKEMIIS EVRNNEVRRR ITILPQEVES LAQQVGNQND ASTELNLSHI LIPLPENPTS DQVNEAESQA RAIVDQARNG ADFGKLAIAH SADQQALNGG QMGWGRIQEL PGIFAQALST AKKGDIVGPI RSGVGFHILK VNDLRGESKN ISVTEVHARH ILLKPSPIMT DEQARVKLEQ IAADIKSGKT TFAAAAKEFS QDPGSANQGG DLGWATPDIF DPAFRDALTR LNKGQMSAPV HSSFGWHLIE LLDTRNVDKT DAAQKDRAYR MLMNRKFSEE AASWMQEQRA SAYVKILSN
    • 分子量47 kDa
    • 氨基酸21 to 428
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab99241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity is > 450 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant surA protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • 应用

    SDS-PAGE

    Mass Spectrometry

    Functional Studies

  • 质谱法
    MALDI-TOF
  • 纯度> 95 % SDS-PAGE.
    ab99241 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Chaperone surA
    • ECK0054
    • JW0052
    • Peptidyl prolyl cis trans isomerase
    • PPIase SurA
    • Rotamase surA
    • Survival protein A
    see all
  • 相关性surA is a chaperone involved in the correct folding and assembly of outer membrane proteins, such as ompA, ompF and lamB. It recognizes specific patterns of aromatic residues and the orientation of their side chains, which are found more frequently in integral outer membrane proteins. surA may act in both early periplasmic and late outer membrane-associated steps of protein maturation and is essential for the survival of E.coli in stationary phase.
  • 细胞定位Periplasm. Note: Is capable of associating with the outer membrane.

Recombinant E. coli surA protein 图像

  • 15% SDS-PAGE showing ab99241 at approximately 47.3kDa (3µg).

Recombinant E. coli surA protein (ab99241)参考文献

ab99241 has not yet been referenced specifically in any publications.

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