Recombinant E. coli RNase H protein (ab113149)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP0A7Y4
    • 种属Escherichia coli
    • 分子量20 kDa including tags
    • 氨基酸1 to 155
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab113149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF
  • 纯度> 95 % SDS-PAGE.
    ab113149 was purified using conventional chromatography techniques.
  • 形式Liquid
  • 补充说明


  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 10% Glycerol, 0.04% DTT

常规信息

  • 别名
    • Ribonuclease H
    • Ribonuclease HI
    • RNase H
    • RNase HI
    • rnhA
    see all
  • 相关性RNase H from E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA in RNA:DNA duplexes to generate products with 3'-hydroxyl and 5'-phosphate ends.1,2,3 RNase H degrades only the RNA component of the DNA-RNA hybrid (RNA that is hydrogen bonded to a complementary DNA strand). Other enzymes in E. coli which degrade RNA in the DNA-RNA hybrid are DNA polymerase I and exonuclease III, but these degrade either the RNA or DNA of the hybrids. Ribonuclease H will not cleave single-stranded or double-stranded DNA or RNA.1,2
  • 细胞定位Cytoplasmic

Recombinant E. coli RNase H protein 图像

  • 15% SDS-PAGE showing ab113149 (3 µg) at approximately 20 kDa.

Recombinant E. coli RNase H protein (ab113149)参考文献

ab113149 has not yet been referenced specifically in any publications.

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