Recombinant E. coli hchA protein (ab106879)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP31658
    • 种属Escherichia coli
    • 序列MGSSHHHHHHSSGLVPRGSHMTVQTSKNPQVDIAEDNAFFPSEYSLSQYT SPVSDLDGVDYPKPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLL PLYHLHAAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNP KKLADVVASLNADSEYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRF VISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTPEIGYMPGHLTWY FGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQEMLAA YAG
    • 分子量33 kDa including tags
    • 氨基酸1 to 283
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab106879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF
  • 纯度> 95 % SDS-PAGE.
    ab106879 was purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

常规信息

  • 别名
    • Chaperone protein hchA
    • EcHsp31
    • ECK1963
    • hchA
    • HCHA_ECOLI
    • Hsp31
    • Hsp31 molecular chaperone
    • JW1950
    • YedU
    • YzzC
    see all
  • 功能Uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress. It rapidly releases them once stress has abated.
  • 序列相似性Belongs to the peptidase C56 family. HchA subfamily.
  • 结构域Consists of a large A domain and a smaller P domain connected by a linker. The thermally induced motion of the flexible linker-loop region leads to the uncovering of a high-affinity substrate-binding site that is essential to capture nonnative proteins at high temperatures.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant E. coli hchA protein 图像

  • 15% SDS-PAGE analysis of 3 µg ab106879.

Recombinant E. coli hchA protein (ab106879)参考文献

ab106879 has not yet been referenced specifically in any publications.

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