重组E. coli GrpE蛋白(ab63839)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE
描述
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产品名称
重组E. coli GrpE蛋白 -
纯度
> 90 % SDS-PAGE.
ab63839 was overexpressed in E.coli and purified by using the conventional column chromatography techniques. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Escherichia coli -
序列
MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA
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描述
重组E. coli GrpE蛋白
技术指标
Our Abpromise guarantee covers the use of ab63839 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.242% Tris, 0.58% Sodium chloride
常规信息
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别名
- Heat shock protein B25.3
- HSP 70 cofactor
- HSP24
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相关性
GrpE is a so-called co-chaperone because it is known to assist the DnaK (Hsp70) protein to effectively carry out DnaK-dependent chaperone activity (i.e. protein folding, protein transport, disaggregation of heat inactivated proteins, activation of mutant protein). GrpE acts as a nucleotide exchange factor for DnaK, the main Hsp70 protein in bacteria, accelerating ADP/ATP exchange by several orders of magnitude. Unfolded proteins bind initially to dnaJ; upon interaction with the dnaJ-bound protein, dnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from dnaK; ATP binding to dnaK triggers the release of the substrate protein, thus completing the reaction cycle. GrpE may also function as a thermosensor. GrpE is a homodimer, each subunit containing three structural domains: a N-terminal unordered segment, two long coils and a C-terminal globular domain formed by a four-helix bundle, and a beta-subdomain. -
细胞定位
Cytoplasmic
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab63839 被引用在 1 文献中.
- Goemans CV et al. CnoX Is a Chaperedoxin: A Holdase that Protects Its Substrates from Irreversible Oxidation. Mol Cell 70:614-627.e7 (2018). PubMed: 29754824