Recombinant E. coli Glucose 6 Phosphate Dehydrogenase protein (ab87414)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Escherichia coli
    • 序列MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

技术指标

Our Abpromise guarantee covers the use of ab87414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 90 % SDS-PAGE.
    Purified by conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: 10% Glycerol, 50mM MES, 2mM EDTA, 0.5mM DTT, 0.1mM PMSF, pH 6

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • G6PD
    • G6PD_HUMAN
    • G6PD1
    • G6pdx
    • Glucose 6 phosphate 1 dehydrogenase
    • Glucose 6 phosphate dehydrogenase
    • Glucose 6 phosphate dehydrogenase, G6PD
    • Glucose-6-phosphate 1-dehydrogenase
    • MET19
    • POS10
    • Zwf1p
    see all
  • 功能Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • 组织特异性Isoform Long is found in lymphoblasts, granulocytes and sperm.
  • 通路Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • 疾病相关Anemia, non-spherocytic hemolytic, due to G6PD deficiency
  • 序列相似性Belongs to the glucose-6-phosphate dehydrogenase family.
  • 翻译后修饰Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Information by UniProt

Recombinant E. coli Glucose 6 Phosphate Dehydrogenase protein 图像

  • ab87414 on 15% SDS-PAGE (4µg)

Recombinant E. coli Glucose 6 Phosphate Dehydrogenase protein (ab87414)参考文献

ab87414 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

I can confirm that ab87414 is a recombinant full length E coli Glucose 6 Phosphate Dehydrogenase and it is not tagged.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"