Recombinant E. coli GFP protein (ab119740) (ab119740)


  • 产品名称Recombinant E. coli GFP protein (ab119740)
    参阅全部 GFP 蛋白酶
  • 蛋白长度Full length protein
  • 描述
    Recombinant E. coli GFP protein


  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionB6UPG7
    • 种属Escherichia coli
    • 分子量39 kDa including tags
    • 氨基酸1 to 238
    • 标签His tag N-Terminus


Our Abpromise guarantee covers the use of ab119740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot


  • 纯度> 80 % SDS-PAGE.
    The purity was determined to be 80% by densitometry
  • 形式Liquid
  • Concentration information loading...


  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.002% PMSF, 0.71% Sodium phosphate, 0.005% DTT, 25% Glycerol, 1.75% Sodium chloride


  • 别名
    • GFP
    • Green fluorescent protein
    • yfp
  • 相关性Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.

Recombinant E. coli GFP protein (ab119740) 图像

  • The purity of ab119740 was determined to be >80% by densitometry.

Recombinant E. coli GFP protein (ab119740) (ab119740)参考文献

ab119740 has not yet been referenced specifically in any publications.

Product Wall

The sequence of the protein is at this page,, and alignment of that sequence with A. Victoria shows 99% identity, and with A. coerulescens, 92% identity.