Recombinant E. coli DnaJ protein (ab91598)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Escherichia coli

技术指标

Our Abpromise guarantee covers the use of ab91598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Protein Refolding Assay: Positive Tested positive for its ability to stimulate DnaK's protein-refolding activity in the presence of GrpE.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度> 90 % SDS-PAGE.
    > 90% pure as determined by SDS-PAGE and Western Blot analysis
  • 形式Liquid
  • 补充说明DnaJ has a tendency to aggregate in solution with ionic strength lower than 0.1 M KCl. Where lower salt conditions are required, addition of 0.05% Triton-X100 or Brij 58 is recommended.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 5mM DTT, 25mM HEPES, 200mM Potassium chloride, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Chaperone Hsp40 co chaperone with DnaK
    • Chaperone protein dnaJ
    • ECK0015
    • faa
    • groP
    • grpC
    • Heat shock protein J
    • HSP40
    • JW0014
    see all
  • 相关性The E. coli heat-shock protein DnaJ has been implicated in protein folding and protein complex dissociation. DnaJ, Heat shock protein, functions in association with DnaK(Hsp70) molecular chaperone to facilitate protein folding. p70 chaperone. DnaJ plays a key role in the chaperone reaction by stimulating the ATPase activity and activating the substrate binding of Hsp70. DnaJ consists of four domains that are N-terminal 76 amino acid J-domain, G/F domain, zinc-binding cystein rich CR-domain, C-terminal CTD-domain and they are conserved to various degrees among the homologues.
  • 细胞定位Cytoplasmic

Recombinant E. coli DnaJ protein 图像

  • SDS-PAGE Analysis:
    Lane 1: Molecular weight markers
    Lane 2: 0.5 µg ab91598
    Lane 3: 1 µg ab91598
    Lane 4: 2 µg ab91598
    Lane 5: 5 µg ab91598
  • All lanes : DnaJ Polyclonal Antibody

    Lane 1 : Molecular weight markers
    Lane 2 : ab91598 at 0.1 µg

Recombinant E. coli DnaJ protein (ab91598)参考文献

ab91598 has not yet been referenced specifically in any publications.

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