Recombinant DUT protein (ab68279)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli

技术指标

Our Abpromise guarantee covers the use of ab68279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific Activity: 1MIU/mg. Unit Defenition: One unit of enzyme catalyzes hydrolysis of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade.
  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • 形式Liquid
  • 补充说明

    The enzymes of Pyrococcus furiosus are extremely thermostable. Consequently the DNA Polymerase from Pyrococcus furiosus (also known as Pfu DNA polymerase) can be used in the polymerase chain reaction (PCR) DNA amplification process. It hydrolyzes dUTP to dUMP and pyrophosphate, simultaneously reducing contaminating dUTP levels and providing the dUMP for dTTP biosynthesis.

    Enhancing PCR amplication: 50µl of Pfu PCR reaction system with 1-3u of dUTPase to amplify genomic DNA target up to 15-19 kb in length.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol (at concentration of 10 u/µl of the enzyme), 0.1% Tween 20 and 50, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.1mM EDTA, 0.1% Nonidet P40, pH 8.2

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform
    • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
    • Deoxyuridine triphosphatase
    • dut
    • DUT_HUMAN
    • dUTP nucleotidohydrolase
    • dUTP pyrophosphatase
    • dUTPase
    • FLJ20622
    see all
  • 功能This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
  • 组织特异性Found in a variety of tissues.
  • 通路Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
  • 序列相似性Belongs to the dUTPase family.
  • 翻译后修饰Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
  • 细胞定位Mitochondrion and Nucleus.
  • Information by UniProt

Recombinant DUT protein (ab68279)参考文献

ab68279 has not yet been referenced specifically in any publications.

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