The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity: 1MIU/mg. Unit Defenition: One unit of enzyme catalyzes hydrolysis of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade.
% SDS-PAGE. Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
The enzymes of Pyrococcus furiosus are extremely thermostable. Consequently the DNA Polymerase from Pyrococcus furiosus (also known as Pfu DNA polymerase) can be used in the polymerase chain reaction (PCR) DNA amplification process. It hydrolyzes dUTP to dUMP and pyrophosphate, simultaneously reducing contaminating dUTP levels and providing the dUMP for dTTP biosynthesis.
Enhancing PCR amplication: 50µl of Pfu PCR reaction system with 1-3u of dUTPase to amplify genomic DNA target up to 15-19 kb in length.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 50% Glycerol (at concentration of 10 u/µl of the enzyme), 0.1% Tween 20 and 50, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.1mM EDTA, 0.1% Nonidet P40, pH 8.2
This product is an active protein and may elicit a biological response in vivo, handle with caution.
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.