Recombinant Cytomegalovirus Glycoprotein B (ab43040)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 额外的序列信息
      The protein contains the Cytomegalovirus (CMV) gB immunodominant regions.

技术指标

Our Abpromise guarantee covers the use of ab43040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Immunoreactive with sera of CMV infected individuals.
  • 应用

    ELISA

    Western blot

  • 纯度
    > 95 % SDS-PAGE.
    Sepharose derived purification.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 25mM Tris HCl, 1mM EDTA, pH 7.2

常规信息

  • 别名
    • CMV glycoprotein B
    • Glycoprotein B
    • HCMV
    • HHV5 glycoprotein B
    • UL55
    see all
  • 相关性
    Human cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB.
  • 细胞定位
    Virus envelope.

文献

ab43040 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your enquiry. This product was made from a 57 amino acid long sequence and a GST fusion. The total molecular weight should be 32.2kDA. Therefore, please rest assured that the band you are seeing is indeed the Cytomegalovirus Glycoprot...

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This protein (ab43040: Active Cytomegalovirus Glycoprotein B protein fragment) was developed for research purposes for ELISA and Western blot applications only - but not for diagnostic or internal use in vivo. Therefore, no endotoxin tests we...

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Thank you for your inquiry.

I can confirm that the immunogen for ab43040 is from within the amino acid range 11-67. I am sorry the exact sequence is proprietary.

Unfortunately, we are not able to release the immunogen sequence for...

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Thank you for supplying that information.


I have been in contact with the lab and they suggested that maybe the PAGE sample was heated too long (5 minutes) prior to loading. It is most likely that a specific smallmolecular weight amino a...

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Thank you for contacting Abcam.


I have been in contact with the lab about your enquiry as to whether a doublet was expected on a western blot, when you ran ab43040 on a western blot. They have asked for some more information:

...

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Sorry for the late reply. The protein contains the CMV gB immunodominant regions without the signal peptide. I hope this is helpful. Please contact us again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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