Recombinant Cytomegalovirus Glycoprotein B (ab43040)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 额外的序列信息The protein contains the Cytomegalovirus (CMV) gB immunodominant regions.

技术指标

Our Abpromise guarantee covers the use of ab43040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Immunoreactive with sera of CMV infected individuals.
  • 应用

    ELISA

    Western blot

  • 纯度> 95 % SDS-PAGE.
    Sepharose derived purification.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 25mM Tris HCl, 1mM EDTA, pH 7.2

常规信息

  • 别名
    • CMV glycoprotein B
    • Glycoprotein B
    • HCMV
    • HHV5 glycoprotein B
    • UL55
    see all
  • 相关性Human cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB.
  • 细胞定位Virus envelope.

Recombinant Cytomegalovirus Glycoprotein B (ab43040)参考文献

ab43040 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. This product was made from a 57 amino acid long sequence and a GST fusion. The total molecular weight should be 32.2kDA. Therefore, please rest assured that the band you are seeing is indeed the Cytomegalovirus Glycoprot...

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This protein (ab43040: Active Cytomegalovirus Glycoprotein B protein fragment) was developed for research purposes for ELISA and Western blot applications only - but not for diagnostic or internal use in vivo. Therefore, no endotoxin tests we...

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Thank you for your inquiry.

I can confirm that the immunogen for ab43040 is from within the amino acid range 11-67. I am sorry the exact sequence is proprietary.

Unfortunately, we are not able to release the immunogen sequence for...

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Thank you for supplying that information.


I have been in contact with the lab and they suggested that maybe the PAGE sample was heated too long (5 minutes) prior to loading. It is most likely that a specific smallmolecular weight amino a...

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Thank you for contacting Abcam.


I have been in contact with the lab about your enquiry as to whether a doublet was expected on a western blot, when you ran ab43040 on a western blot. They have asked for some more information:

...

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Sorry for the late reply. The protein contains the CMV gB immunodominant regions without the signal peptide. I hope this is helpful. Please contact us again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"