The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生物活性The biological activity was measured in a cell activation assay using the Griess reaction as an indicator of nitric oxide production by bovine PBMC. The ED50 for this effect is typically 50 - 60 ug/ml.
% SDS-PAGE. ab87928 was purified using Ion-exchange chromatography.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
复溶Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C.
功能Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
组织特异性Released primarily from activated T lymphocytes.
疾病相关In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
序列相似性Belongs to the type II (or gamma) interferon family.
翻译后修饰Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.