重组牛Hsc70蛋白(ab91596)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
描述
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产品名称
重组牛Hsc70蛋白
参阅全部 Hsc70 蛋白酶 -
生物活性
ATPase Activity Assay: Positive
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纯度
> 80 % SDS-PAGE.
Purified by Multi-step chromatography -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Cow
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技术指标
Our Abpromise guarantee covers the use of ab91596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
Functional Studies
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形式
Liquid -
补充说明
Endotoxin Concentration: < 50 EU/mg as determined by Limulus Amebocyte Lysate (LAL) gel clot assay -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.363% Tris, 0.0292% EDTA, 1.74% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- 2410008N15Rik
- Constitutive heat shock protein 70
- Epididymis luminal protein 33
see all -
功能
Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the heat shock protein 70 family. -
结构域
The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
细胞定位
Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab91596 尚未被引用在任何文献中。